Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Transcriptomic landscape reveals germline potential of porcine skin-derived multipotent dermal fibroblast progenitors

doi: 10.1007/s00018-023-04869-7

Figure Lengend Snippet: Activation of MAPK signaling pathway promoted the proliferation of PGCLCs. a Schematic diagram of the research design. b Bright field of cultures with 400 mIU LH, 25 μM MLT, or 5 μM RA for 16 days. Scale bar, 100 μm. c Flow cytometry analysis of SOX17 (FITC) and PCNA (CY3) double staining with 400 mIU LH, 25 μM MLT, or 5 μM RA. d Correlation matrix heatmap for control group, LH group, and MLT group. e The KEGG pathway enrichment of DEGs for LH vs control group and MLT vs control. f Venn diagram showed the number of enriched genes in MAPK signaling pathway for LH vs control group and MLT vs control. g Heatmap analysis of DEGs associated with cell proliferation and cell cycle for LH vs control group and MLT vs control. h The protein levels of the p‐MEK (MEK) and p‐ERK (ERK) detected by WB in culturing with LH, MLT, or RA groups for 16 days. i The expression levels of proliferation-related genes were detected by RT-qPCR in culturing with LH, MLT, or RA groups for 16 days. j Bright field of 10 μM U0126 culture for 16 days. Scale bar, 100 μm. k Flow cytometry analysis of SOX17 (FITC) staining with 10 μM U0126. The results are presented as mean ± SD. All the experiments were repeated at least three times. *0.01 < P < 0.05; **P < 0.01

Article Snippet: The reaction was performed by Roche 480 Light Cycler RT-qPCR instrument (Roche, Germany) as previously described [ 36 ].

Techniques: Activation Assay, Flow Cytometry, Double Staining, Expressing, Quantitative RT-PCR, Staining